Derivatives of yeast tRNA(Tyr) lacking in the anticodon-arm (and D-arm) were constructed by a combination of partial digestion with RNase T1 and joining with T4 RNA ligase. Aminoacylation analyses of these derivatives ("3/4 molecule" or "1/2 molecule") showed that sufficient information for binding to TyrRS is contained mainly in the aminoacyl-stem (and T psi C-arm) of yeast tRNA(Tyr), but further information, possibly in the anticodon-arm, is necessary for efficient acceptance of tyrosine.