Allocation of Fe and ferric chelate reductase activities in mesophyll cells of barley and sorghum under Fe-deficient conditions

Abstract

Although the photosynthetic apparatus requires large amounts of Fe, the adaptive mechanisms of mesophyll cells for Fe acquisition under Fe-deficient conditions are unknown. Barley and sorghum, which are tolerant and susceptible to Fe deficiency, respectively, have similar Fe and chlorophyll contents in their leaves. However, the Fe-deficient barley photosynthetic apparatus was functional while that of sorghum was not. We show that barley preferentially allocates Fe to thylakoid membranes under Fe-deficient conditions. On the other hand, in sorghum, the proportion of leaf Fe allocated to thylakoids was not altered by Fe deficiency. The relationship between the maintenance of photosynthesis and light-dependent ferric chelate reductase activity on plasma membranes and chloroplast envelopes is also discussed.