Objective: We analyzed the prokaryotic microbial diversity of Eryuan Niujie hot spring, in Yunnan Province, to enrich our knowledge about thermo-stable microbes.
Method: We constructed bacterial and archaeal 16S rRNA gene libraries, analyzed the sequences and constructed phylogenetic trees to learn the prokaryotic microbial diversity.
Result: The majority of the prokaryotic microbes in this hot spring were bacteria, while beta-Proteobacteria was the most abundant, next were Bacteroidetes and Chlorobi; the abundance and diversity of archaea were both less than that of bacteria, including Euryarchaeota and Crenarchaeota, while Euryarchaeota was the most abundant.