Identification of a linear epitope on the capsid protein of classical swine fever virus

Virus Res. 2011 Mar;156(1-2):134-40. doi: 10.1016/j.virusres.2011.01.009. Epub 2011 Jan 19.

Abstract

The capsid (C) protein of Classical swine fever virus (CSFV) is proposed to play an essential role in the replication and translation of the viral RNA. In this study, a monoclonal antibody (mAb) directed against the C protein was generated with the recombinant C protein expressed in Escherichia coli as immunogen. IFA and IPMA analysis showed that the native C protein of CSFV virions was reactive to the mAb. By truncating the C protein, we identified a linear epitope recognized by the mAb, corresponding to amino acids (61)TQDGLYHNKN(70) of the CSFV C protein, which is well conserved among pestiviruses. Laser confocal analysis showed that the C protein mainly locates in the cellular nucleoplasm and nucleolus of PK-15 cells. The results have implications for further study of CSFV replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Capsid Proteins / genetics
  • Capsid Proteins / immunology*
  • Cells, Cultured
  • Classical Swine Fever Virus / immunology*
  • Epitope Mapping
  • Epitopes / chemistry
  • Epitopes / immunology*
  • Intracellular Space / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Protein Transport
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Sequence Alignment
  • Swine

Substances

  • Antibodies, Monoclonal
  • Capsid Proteins
  • Epitopes
  • Recombinant Fusion Proteins