Objective: To clone Aralia elata squalene synthase gene (designated as AeSS) and construct plant expression vector for transgenic research.
Methods: Isolated squalene synthase from Aralia elata with specific primers by RT-PCR and inserted AeSS gene into the plant expression vector pBI121.
Results: The full-length cDNA of AeSS (Genebank accession Number: GU354313) was 1 261 bp and contained a 1 245 bp open reading frame (ORF) encoding a polypeptide of 414 amino acids. The plant expression vector pAeSS was constructed by inserted AeSS gene into the downstream of 35 S promoter of plant expression vector pBI121.
Conclusion: AeSS gene was cloned and plant expression vector was constructed for future research.