Crystallization of recombinant bifunctional nuclease TBN1 from tomato

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):124-8. doi: 10.1107/S1744309110048177. Epub 2010 Dec 23.

Abstract

The endonuclease TBN1 from Solanum lycopersicum (tomato) was expressed in Nicotiana benthamiana leaves and purified with suitable quality and in suitable quantities for crystallization experiments. Two crystal forms (orthorhombic and rhombohedral) were obtained and X-ray diffraction experiments were performed. The presence of natively bound Zn2+ ions was confirmed by X-ray fluorescence and by an absorption-edge scan. X-ray diffraction data were collected from the orthorhombic (resolution of 5.2 Å) and rhombohedral (best resolution of 3.2 Å) crystal forms. SAD, MAD and MR methods were applied for solution of the phase problem, with partial success. TBN1 contains three Zn2+ ions in a similar spatial arrangement to that observed in nuclease P1 from Penicillium citrinum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Crystallization
  • Crystallography, X-Ray
  • Deoxyribonucleases / chemistry*
  • Deoxyribonucleases / genetics
  • Ions / chemistry
  • Molecular Sequence Data
  • Plant Proteins / chemistry*
  • Plant Proteins / genetics
  • Protein Conformation
  • Recombinant Proteins / chemistry*
  • Recombinant Proteins / genetics
  • Solanum lycopersicum / chemistry*
  • Solanum lycopersicum / genetics
  • Zinc / chemistry

Substances

  • Ions
  • Plant Proteins
  • Recombinant Proteins
  • Deoxyribonucleases
  • Zinc