1H, 13C and 15N resonances of the AlgE62 subunit from Azotobacter vinelandii mannuronan C5-epimerase

Trygve Andreassen; Edith Buchinger; Gudmund Skjåk-Bræk; Svein Valla; Finn L Aachmann

doi:10.1007/s12104-010-9288-8

1H, 13C and 15N resonances of the AlgE62 subunit from Azotobacter vinelandii mannuronan C5-epimerase

Biomol NMR Assign. 2011 Oct;5(2):147-9. doi: 10.1007/s12104-010-9288-8. Epub 2010 Dec 28.

Authors

Trygve Andreassen¹, Edith Buchinger, Gudmund Skjåk-Bræk, Svein Valla, Finn L Aachmann

Affiliation

¹ Department of Biotechnology, Norwegian University of Science and Techology, Sem Sælands vei 6/8, 7491, Trondheim, Norway.

Abstract

The 17.7 kDa R2 module from Azotobacter vinelandii mannronan C5-epimerase AlgE6 has been isotopically labeled ((13)C,(15)N) and recombinantly expressed. Here we report the (1)H, (13)C, (15)N resonance assignment of AlgE6R2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azotobacter vinelandii / enzymology*
Carbohydrate Epimerases / chemistry*
Carbon Isotopes
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular*
Recombinant Proteins / chemistry

Substances

Carbon Isotopes
Nitrogen Isotopes
Recombinant Proteins
Carbohydrate Epimerases
mannuronan c-5-epimerase