Modulation of i-motif thermodynamic stability by the introduction of UNA (unlocked nucleic acid) monomers

Anna Pasternak; Jesper Wengel

doi:10.1016/j.bmcl.2010.11.106

Modulation of i-motif thermodynamic stability by the introduction of UNA (unlocked nucleic acid) monomers

Bioorg Med Chem Lett. 2011 Jan 15;21(2):752-5. doi: 10.1016/j.bmcl.2010.11.106. Epub 2010 Nov 26.

Authors

Anna Pasternak¹, Jesper Wengel

Affiliation

¹ Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.

PMID: 21185179
DOI: 10.1016/j.bmcl.2010.11.106

Abstract

The influence of acyclic RNA derivatives, UNA (unlocked nucleic acid) monomers, on i-DNA thermodynamic stability has been investigated. The 22nt human telomeric fragment was chosen as the model sequence for stability studies. UNA monomers modulate i-motif stability in a position-depending manner. The largest destabilization is observed for position C14, while UNA placed in position A12 causes significant increase of i-DNA thermodynamic stability. CD curves of UNA-modified variants imply no structural changes relative to the native i-motif.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Circular Dichroism
DNA / chemistry*
Humans
Nucleic Acid Conformation
RNA / chemistry*
Telomere / chemistry
Thermodynamics

Substances

RNA
DNA