A new methodology for rapid detection of Lactobacillus delbrueckii subsp. bulgaricus based on multiplex PCR

Anastasios Nikolaou; Georgia Saxami; Yiannis Kourkoutas; Alex Galanis

doi:10.1016/j.mimet.2010.12.010

A new methodology for rapid detection of Lactobacillus delbrueckii subsp. bulgaricus based on multiplex PCR

J Microbiol Methods. 2011 Feb;84(2):362-4. doi: 10.1016/j.mimet.2010.12.010. Epub 2010 Dec 21.

Authors

Anastasios Nikolaou¹, Georgia Saxami, Yiannis Kourkoutas, Alex Galanis

Affiliation

¹ Applied Microbiology and Molecular Biotechnology Research Group, Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.

PMID: 21182878
DOI: 10.1016/j.mimet.2010.12.010

Abstract

In this study we present a novel multiplex PCR assay for rapid and efficient detection of Lactobacillus delbrueckii subsp. bulgaricus. The accuracy of our method was confirmed by the successful identification of L. delbrueckii subsp. bulgaricus in commercial yoghurts and food supplements and it may be readily applied to the food industry.

Publication types

Evaluation Study

MeSH terms

Bacteriological Techniques / methods*
Dietary Supplements / microbiology*
Humans
Lactobacillus delbrueckii / classification*
Lactobacillus delbrueckii / genetics
Lactobacillus delbrueckii / isolation & purification*
Polymerase Chain Reaction / methods*
Yogurt / microbiology*