Identification of carotid 'vulnerable plaque' by contrast-enhanced ultrasonography: correlation with plaque histology, symptoms and cerebral computed tomography

Eur J Vasc Endovasc Surg. 2011 Feb;41(2):238-48. doi: 10.1016/j.ejvs.2010.11.002. Epub 2010 Dec 8.

Abstract

Introduction: Indication to carotid revascularisation is commonly determined by percent of stenosis as well as neurological symptoms and clinical conditions. High plaque embolic potential is defined as 'vulnerability'; however, its characterisation is not universally used for carotid revascularisation. We investigated the role of contrast-enhanced ultrasonography (CEUS) to identify carotid vulnerable plaque.

Methods: Patients undergoing carotid endarterectomy were preoperatively evaluated by cerebral computed tomography (CT) scan and CEUS. Contrast microbubbles detected within the plaque indicated neovascularisation and were quantified by decibel enhancement (dB-E). Plaques were histologically evaluated for five features: (microvessel density, fibrous cap thickness, extension of calcification, inflammatory infiltrate and lipid core) and blindly scored 1-5 to assess plaque vulnerability. Analysis of variance (ANOVA), Fisher's and Student's t-test were used to correlate patients' characteristics, histological features and dB-E.

Results: In 22 patients, dB-E (range 2-7.8, mean 4.85 ± 1.9 SD) was significantly greater in symptomatic (7.40 ± 0.5) vs. asymptomatic (3.5 ± 1.4) patients (p = 0.002). A higher dB-E was significantly associated with thinner fibrous cap (<200 μm, 5.96 ± 1.5 vs. 3 ± 1, p = 0.01) and greater inflammatory infiltrate (3.2 ± 0.9 vs. 6.4 ± 1.2, p = 0.03). Plaques with vulnerability score of 5 had significantly higher dB-E compared with those with vulnerability score of 1 (7.6 ± 0.2 vs. 2.5 ± 0.6, respectively, p = 0.001). Preoperative ipsilateral embolic lesions at CT were correlated with higher dB-E (5.96 ± 1.5 vs. 3.0 ± 1.0, p = 0.01).

Conclusion: CEUS with dB-E is indicative of the extent of plaque neovascularisation. It can be used therefore as a marker for vulnerable plaque.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Analysis of Variance
  • Calcinosis / pathology
  • Carotid Stenosis / complications
  • Carotid Stenosis / diagnostic imaging*
  • Carotid Stenosis / pathology
  • Carotid Stenosis / surgery
  • Cerebral Angiography / methods*
  • Contrast Media*
  • Endarterectomy, Carotid
  • Female
  • Fibrosis
  • Humans
  • Immunohistochemistry
  • Intracranial Embolism / diagnostic imaging*
  • Intracranial Embolism / etiology
  • Ischemic Attack, Transient / diagnostic imaging*
  • Ischemic Attack, Transient / etiology
  • Italy
  • Lipids / analysis
  • Male
  • Microbubbles
  • Neovascularization, Pathologic / pathology
  • Predictive Value of Tests
  • Prognosis
  • Severity of Illness Index
  • Stroke / diagnostic imaging*
  • Stroke / etiology
  • Tomography, X-Ray Computed*
  • Ultrasonography

Substances

  • Contrast Media
  • Lipids