Abstract
The CEM-ON malignant T cell line and long-term cultured normal T cells can be induced to release CSF-1 in their culture supernatants. Chemical inducers (PMA + A23187) and, more interestingly, cytokines (tumor necrosis factor alpha (TNF alpha) and interleukin-1 alpha (IL-1 alpha)), as well as physiological (antigen + IL-2) or specific (anti-CD3 + IL-2 or PMA) stimuli, lead to rapid transient colony-stimulating factor-1 (CSF-1) gene expression and production of biologically active CSF-1. These data suggest that CSF-1 may play a role in the early phases of immune response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Differentiation, T-Lymphocyte / analysis
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Blotting, Northern
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Calcimycin / pharmacology
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Cells, Cultured
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Colony-Forming Units Assay
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Colony-Stimulating Factors / biosynthesis*
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Colony-Stimulating Factors / genetics
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Humans
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Interleukin-1 / pharmacology
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Leukemia, T-Cell / immunology
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Leukemia, T-Cell / metabolism*
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Macrophage Colony-Stimulating Factor
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Monocytes / cytology
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Phenotype
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RNA, Messenger / metabolism
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T-Lymphocytes / drug effects
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism*
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / immunology
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Tumor Cells, Cultured / metabolism
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Antigens, Differentiation, T-Lymphocyte
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Colony-Stimulating Factors
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Interleukin-1
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Calcimycin
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Macrophage Colony-Stimulating Factor
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Tetradecanoylphorbol Acetate