Maintenance of undifferentiated state of human embryonic stem cells in chemical defined medium at high clone density without exogenous cell factors

Shuwei Luo; Ge Lin; Zhen Sun; Pingyuan Xie; Tiancheng Liu; Guangxiu Lu

doi:10.3969/j.issn.1672-7347.2010.11.

Maintenance of undifferentiated state of human embryonic stem cells in chemical defined medium at high clone density without exogenous cell factors

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 Nov;35(11):1123-8. doi: 10.3969/j.issn.1672-7347.2010.11..

Authors

Shuwei Luo¹, Ge Lin, Zhen Sun, Pingyuan Xie, Tiancheng Liu, Guangxiu Lu

Affiliation

¹ Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha 410078, China.

PMID: 21131732
DOI: 10.3969/j.issn.1672-7347.2010.11.

Abstract

Objective: To establish human embryonic stem cells (hESCs) feeder-independent and cell factor-free culture system.

Methods: Effect of high and low clone densities of hESCs culture was compared and impact of the clone densities to hESCs culture media was analyzed.

Results: HESCs could maintain their undifferentiated states at high clone density (34 clones/cm²) without cell factors. At the same time, the bone morphology protein (BMP)-like induction of N2 and B27 supplements (NB) medium could be modulated by the clone density, and high level of BMP-like induction was accompanied by high clone density.

Conclusion: High clone density of hESCs can change the environments by themselves to maintain the undifferentiated states, which provides a new clue to explore the mechanism of undifferentiated states of hESCs and simplify the culture medium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bone Morphogenetic Proteins / pharmacology
Cell Culture Techniques / methods*
Cell Differentiation / physiology*
Cloning, Molecular
Culture Media*
Embryonic Stem Cells / cytology*
Gene Expression Regulation, Developmental
Humans

Substances

Bone Morphogenetic Proteins
Culture Media