Time-resolved X-ray scattering and RNA folding

Lois Pollack; Sebastian Doniach

doi:10.1016/S0076-6879(09)69012-1

Time-resolved X-ray scattering and RNA folding

Methods Enzymol. 2009:469:253-68. doi: 10.1016/S0076-6879(09)69012-1. Epub 2009 Nov 17.

Authors

Lois Pollack¹, Sebastian Doniach

Affiliation

¹ School of Applied and Engineering Physics, Cornell University, Ithaca, New York, USA.

PMID: 20946793
DOI: 10.1016/S0076-6879(09)69012-1

Abstract

Time-resolved small-angle X-ray scattering (SAXS) reports changes in the global conformation of macromolecules and is thus a valuable probe of structural transitions like RNA folding. Time-resolved SAXS has been applied to study folding of the Tetrahymena ribozyme. This chapter describes the methods that enable acquisition and analysis of time-resolved SAXS data and insights into RNA folding gained from these studies.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Nucleic Acid Conformation
RNA / chemistry*
Scattering, Radiation*
X-Rays

Substances

RNA

Abstract

Publication types

MeSH terms

Substances

Grants and funding