Abstract
We constructed a dominant negative form of human hypoxia-inducible factor (HIF)-2α, HIF-2αDoN, which inhibited HIF transcriptional activity induced by hypoxia and by HIF-2α. HIF-2αDoN formed a complex with HIF-1β and interacted with DNA containing hypoxia response elements (HREs). Thus, the complex appears to inhibit the binding of HIF-2 to HREs, and HIF-2αDoN might provide a useful therapeutic tool for HIF-2α-related diseases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Basic Helix-Loop-Helix Transcription Factors / genetics*
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Basic Helix-Loop-Helix Transcription Factors / metabolism
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Cell Line, Tumor
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Genes, Dominant / genetics*
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HEK293 Cells
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Humans
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Protein Engineering / methods*
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Response Elements / genetics
Substances
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Basic Helix-Loop-Helix Transcription Factors
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Protein Isoforms
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RNA, Messenger
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endothelial PAS domain-containing protein 1