Four new species of Stigmatomyces (Ascomycota, Laboulbeniomycetes, Stigmatomycetinae) parasitic on flies (Diptera) are described. These are S. aphaniosomae, parasitic on an undescribed species of Aphaniosoma (Chyromyidae) from Jordan; S. deemingii, parasitic on Milichia pubescens (Milichiidae) from Yemen; S. macanus, parasitic on Phortica spp. (Drosophilidae) from Taiwan; and S. mosilli, parasitic on Mosillus subsultans (Ephydridae) from Pakistan. Chyromyidae is a new host family for Laboulbeniales, and S. aphaniosomae is the first of the Laboulbeniales to be reported from Jordan.