Protection against increased intestinal permeability and bacterial translocation induced by intestinal obstruction in mice treated with viable and heat-killed Saccharomyces boulardii

Eur J Nutr. 2011 Jun;50(4):261-9. doi: 10.1007/s00394-010-0134-7. Epub 2010 Oct 10.

Abstract

Background: There are substantial evidences suggesting that probiotics can protect the gastrointestinal tract against inflammatory or infectious episodes. The effects of oral treatment with viable or heat-killed cells of Saccharomyces boulardii (Sb) on bacterial translocation, intestinal permeability, histological aspect of the ileum, and some immunological parameters were evaluated in a murine intestinal obstruction (IO) model.

Results: Bacterial translocation and intestinal permeability in the IO group were significantly higher when compared to a Sham group (p < 0.05). Pretreatment with both viable and heat-killed S. boulardii prevented these increases, and the data obtained for IO + Sb and IO + heat-killed Sb groups were similar to those observed in the Sham group (p > 0.05). Histological analysis showed preservation of the ileum mucosa in mice that received both forms of the yeast when compared to the lesions observed in the IO group. The levels of serum interleukin (IL)-10 and intestinal secretory immunoglobulin A (sIgA) were higher in the animals that received both yeast treatments when compared to those from IO and Sham groups.

Conclusion: Oral treatment with viable or heat-killed cells of S. boulardii maintained intestinal integrity and modulated the immune system in a murine IO model, preventing bacterial translocation and intestinal lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Translocation*
  • Eating
  • Escherichia coli / physiology
  • Hot Temperature
  • Ileitis / immunology
  • Ileitis / pathology
  • Ileitis / physiopathology
  • Ileitis / prevention & control*
  • Ileum / immunology
  • Ileum / pathology
  • Immunoglobulin A, Secretory / analysis
  • Inflammation Mediators / blood
  • Intestinal Obstruction / immunology
  • Intestinal Obstruction / pathology
  • Intestinal Obstruction / physiopathology
  • Intestine, Small / immunology
  • Intestine, Small / pathology
  • Intestine, Small / physiopathology*
  • Male
  • Mice
  • Microbial Viability
  • Permeability
  • Probiotics / therapeutic use*
  • Random Allocation
  • Saccharomyces / physiology*
  • Severity of Illness Index
  • Time Factors
  • Weight Gain

Substances

  • Immunoglobulin A, Secretory
  • Inflammation Mediators