Comparison of BinaxNOW Influenza A&B assay and real-time reverse transcription polymerase chain reaction for diagnosis of influenza A pandemic (H1N1) 2009 virus infection in adult patients

Diagn Microbiol Infect Dis. 2010 Dec;68(4):456-8. doi: 10.1016/j.diagmicrobio.2010.07.010. Epub 2010 Sep 29.

Abstract

The BinaxNOW Influenza A&B assay was evaluated for the diagnosis of influenza A pandemic (H1N1) 2009 virus infection in 354 adult patients. The sensitivity, specificity, and positive and negative predictive values were 32%, 100%, 100%, and 67%, respectively. The analytic sensitivity of the assay was log₁₀5.0 tissue culture infective dose (TCID)₅₀/mL.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Viral / blood*
  • DNA Primers
  • Humans
  • Immunoassay / methods*
  • Influenza A Virus, H1N1 Subtype / genetics
  • Influenza A Virus, H1N1 Subtype / immunology*
  • Influenza A Virus, H1N1 Subtype / isolation & purification
  • Influenza B virus / immunology
  • Influenza, Human / diagnosis*
  • Influenza, Human / virology
  • Middle Aged
  • Nasopharynx / virology
  • Oropharynx / virology
  • Pandemics
  • Predictive Value of Tests
  • RNA, Viral / analysis*
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Young Adult

Substances

  • Antibodies, Viral
  • DNA Primers
  • RNA, Viral