Undetectable HIV-1 RNA load with the Cobas TaqMan v1.0 in a patient diagnosed at the time of primary HIV-1 infection

J Med Virol. 2010 Nov;82(11):1816-8. doi: 10.1002/jmv.21827.

Abstract

Diagnosis of primary HIV-1 infection is challenging due to the presence of a serological window; thus, HIV-1-RNA quantitation and/or measurement of p24 antigenemia are recommended in such cases. A patient was diagnosed at the time of primary HIV-1 infection, he harbored a CFR02_AG subtype virus; quantitation of plasma HIV-1-RNA yielded an undetectable result according to one commercial assay, while HIV-1-RNA was detectable when measured with three other assays.

Publication types

  • Case Reports

MeSH terms

  • HIV Infections / diagnosis*
  • HIV Infections / virology
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • HIV-1 / physiology
  • Humans
  • Male
  • Polymerase Chain Reaction / methods*
  • RNA, Viral / blood*
  • Reagent Kits, Diagnostic*
  • Sensitivity and Specificity
  • Taq Polymerase*
  • Viral Load*

Substances

  • RNA, Viral
  • Reagent Kits, Diagnostic
  • Taq Polymerase