Selection of naturally occurring autoantibodies to interleukin-18 from phage display library

Hum Antibodies. 2010;19(2-3):71-8. doi: 10.3233/HAB-2010-0230.

Abstract

Four unique phage single-chain variable fragments (scFvs) to recombinant human interleukin 18 (IL-18) has been selected from a naïve combinatorial library of human scFvs. Binding of unique phage antibodies with IL-18 was tested by ELISA and Western-blotting. No cross reactivity with tumor necrosis factor α, interferons α and γ was shown for the selected antibodies. The gene segments encoding V(D)J regions of selected antibodies exhibited a high degree of homology to germline genes, therefore we suggest that the selected scFv's belong to repertoire of naïve autoantibodies.

MeSH terms

  • Amino Acid Sequence
  • Autoantibodies / genetics
  • Autoantibodies / immunology*
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoglobulin Heavy Chains / chemistry
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Heavy Chains / immunology
  • Immunoglobulin Light Chains / chemistry
  • Immunoglobulin Light Chains / genetics
  • Immunoglobulin Light Chains / immunology
  • Interleukin-18 / genetics
  • Interleukin-18 / immunology*
  • Molecular Sequence Data
  • Peptide Library*
  • Polymorphism, Restriction Fragment Length
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology*
  • Single-Chain Antibodies / genetics
  • Single-Chain Antibodies / immunology*

Substances

  • Autoantibodies
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Interleukin-18
  • Peptide Library
  • Recombinant Proteins
  • Single-Chain Antibodies