Studies on the permeability of potassium ions across the dura and arachnoid mater of the rat spinal cord

J Neurotrauma. 1990 Winter;7(4):257-66. doi: 10.1089/neu.1990.7.257.

Abstract

A combined impounder-surface K+ electrode was developed to measure change in K+ ion concentration of the cerebrospinal fluid (CSF) across the dura and arachnoid maters. To determine whether K+ permeability of the rat dura and arachnoid maters is due to an intrinsic permeability, a study was conducted using an atraumatic laminectomy model. Dorsal laminectomy was performed at T7-8, T12, and L1. Artificial CSF containing 4.2 mM, 24.2 mM, or 54.2 mM of K+ was administered by anterograde subdural infusion into the subarachnoid space from the proximal laminectomy site (T7-8), with effluent overflow at the distal laminectomy site (L1). K+ concentration on the dorsal aspect of the central laminectomy site (T12) was measured. It was found that changes in K+ concentrations of the infused solution were detected by the epidural surface electrode. This suggests that the intact rat spinal cord dura and arachnoid maters may be permeable to K+ in this laminectomy model. This study supports the use of the combined impounder-K+ electrode for measuring changes in K+ ion concentration of the CSF that can result from spinal cord trauma and ischemia.

MeSH terms

  • Adipose Tissue / metabolism
  • Animals
  • Arachnoid / anatomy & histology
  • Arachnoid / metabolism*
  • Coloring Agents
  • Dura Mater / anatomy & histology
  • Dura Mater / metabolism*
  • Electrodes
  • Injections, Epidural
  • Male
  • Perfusion
  • Permeability
  • Potassium / cerebrospinal fluid
  • Potassium / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Spinal Cord / anatomy & histology
  • Spinal Cord / metabolism*
  • Subarachnoid Space / anatomy & histology
  • Subarachnoid Space / metabolism

Substances

  • Coloring Agents
  • Potassium