A facile scanometric strategy for ultrasensitive detection of protein using aptamer-initiated rolling circle amplification

Wei Cheng; Lin Ding; Yunlong Chen; Feng Yan; Huangxian Ju; Yibing Yin

doi:10.1039/c002078h

A facile scanometric strategy for ultrasensitive detection of protein using aptamer-initiated rolling circle amplification

Chem Commun (Camb). 2010 Sep 28;46(36):6720-2. doi: 10.1039/c002078h. Epub 2010 Aug 23.

Authors

Wei Cheng¹, Lin Ding, Yunlong Chen, Feng Yan, Huangxian Ju, Yibing Yin

Affiliation

¹ Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P.R. China.

PMID: 20730209
DOI: 10.1039/c002078h

Abstract

A pragmatic and simple strategy was developed for ultrasensitive detection of protein with good specificity and low matrix effect, which combined aptamer-initiated rolling circle amplification with a Au nanoparticle probe and convenient scanometric readout.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Gold / chemistry
Humans
Magnetics
Metal Nanoparticles / chemistry
Proteins / analysis*
Silver / chemistry
Vascular Endothelial Growth Factor A / analysis

Substances

Aptamers, Nucleotide
Proteins
Vascular Endothelial Growth Factor A
Silver
Gold