Alternative splicing of genes during neuronal differentiation of NT2 pluripotential human embryonal carcinoma cells

FEBS Lett. 2010 Sep 24;584(18):4041-7. doi: 10.1016/j.febslet.2010.08.024. Epub 2010 Aug 20.

Abstract

We analyzed the mRNA diversity of genes after inducing neuronal differentiation in human NT2 teratocarcinoma cells using all-trans retinoic acid (RA). DNA microarray analyses of cells treated with RA identified 358 RA-responsive genes. mRNA diversity analysis revealed that 274 genes produced multiple protein-coding transcripts by alternative splicing. Among these 274 genes, we chose 26 genes that showed AS in their C-terminus and 12 transcription factor genes for further analysis. By using transcript-specific primers, we performed quantitative real-time PCR analysis to examine the expression profiles of all the protein-coding transcripts. Consequently, we identified genes which showed different RA-induced changes in the expression of their protein-coding transcripts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Cell Line, Tumor
  • Gene Expression
  • Humans
  • Neurogenesis / drug effects
  • Neurogenesis / genetics*
  • Oligonucleotide Array Sequence Analysis
  • Pluripotent Stem Cells / metabolism
  • Transcription Factors / genetics
  • Transcription, Genetic
  • Tretinoin / metabolism*
  • Tretinoin / pharmacology

Substances

  • Transcription Factors
  • Tretinoin