Antagonism of the inhibitory effects of transforming growth factor-beta on colony formation of mouse bone marrow cells in vitro by increasing concentrations of growth factors

Ann N Y Acad Sci. 1991:628:52-8. doi: 10.1111/j.1749-6632.1991.tb17222.x.

Abstract

Low concentrations of TGF-beta inhibited the clonal proliferation of three different preparations of mouse bone marrow progenitor cells in vitro. Normal marrow cells responsive to CSF-1; two-day post-fluorouracil cells responsive to CSF-1 plus IL-3 and IL-1 alpha; and regenerating seven-day post-fluorouracil cells responsive to CSF-1 plus IL-3, IL-1 alpha and IL-6 were all severely inhibited by TGF-beta 1; and the inhibitory effects were antagonized by increasing the concentrations of the relevant growth factors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bone Marrow Cells*
  • Cell Division / drug effects
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Growth Substances / pharmacology*
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / drug effects
  • Interleukin-1 / pharmacology
  • Interleukin-3 / pharmacology
  • Interleukin-6 / pharmacology
  • Macrophage Colony-Stimulating Factor / pharmacology
  • Male
  • Mice
  • Mice, Inbred CBA
  • Recombinant Proteins / pharmacology
  • Transforming Growth Factor beta / antagonists & inhibitors
  • Transforming Growth Factor beta / pharmacology*

Substances

  • Growth Substances
  • Interleukin-1
  • Interleukin-3
  • Interleukin-6
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • Macrophage Colony-Stimulating Factor