Preparation of very-high-yield recombinant proteins using novel high-cell-density bacterial expression methods

Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5475. doi: 10.1101/pdb.prot5475.
No abstract available

MeSH terms

  • Colony Count, Microbial / methods*
  • Culture Media / pharmacology
  • Escherichia coli / cytology*
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism*
  • Isopropyl Thiogalactoside / metabolism
  • Recombinant Proteins / biosynthesis*
  • Temperature
  • Time Factors

Substances

  • Culture Media
  • Recombinant Proteins
  • Isopropyl Thiogalactoside