Activation of α7 nicotinic receptors improves phencyclidine-induced deficits in cognitive tasks in rats: implications for therapy of cognitive dysfunction in schizophrenia

Eur Neuropsychopharmacol. 2011 Apr;21(4):333-43. doi: 10.1016/j.euroneuro.2010.06.003. Epub 2010 Jul 13.

Abstract

Rationale: Nicotinic α7 acetylcholine receptors (nAChRs) have been highlighted as a target for cognitive enhancement in schizophrenia.

Aim: To investigate whether the deficits induced by sub-chronic phencyclidine (PCP) in reversal learning and novel object recognition could be attenuated by the selective α7 nAChR full agonist, PNU-282987.

Methods: Adult female hooded-Lister rats received sub-chronic PCP (2mg/kg) or vehicle i.p. twice daily for 7days, followed by 7 days washout. In cohort 1, PCP-treated rats then received PNU-282987 (5, 10, 20mg/kg; s.c.) or vehicle and were tested in the reversal-learning task. In cohort 2, PCP-treated rats received PNU-282987 (10mg/kg; s.c.) or saline for 15days and were tested in the novel object recognition test on day 1 and on day 15, to test for tolerance.

Results: Sub-chronic PCP produced significant deficits in both cognitive tasks (P<0.01-0.001). PNU-282987 attenuated the PCP-induced deficits in reversal learning at 10mg/kg (P<0.01) and 20mg/kg (P<0.001), and in novel object recognition at 10mg/kg on day 1 (P<0.01) and on day 15 (P<0.001).

Conclusions: These data show that PNU-282987 has efficacy to reverse PCP-induced deficits in two paradigms of relevance to schizophrenia. Results further suggest that 15-day once daily dosing of PNU-282987 (10mg/kg s.c.) does not cause tolerance in the rat. This study suggests that activation of α7 nAChRs, may represent a suitable strategy for improving cognitive deficits of relevance to schizophrenia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal / drug effects
  • Benzamides / administration & dosage
  • Benzamides / blood
  • Benzamides / pharmacokinetics
  • Benzamides / therapeutic use*
  • Bridged Bicyclo Compounds / administration & dosage
  • Bridged Bicyclo Compounds / blood
  • Bridged Bicyclo Compounds / pharmacokinetics
  • Bridged Bicyclo Compounds / therapeutic use*
  • Cognition / drug effects
  • Cognition Disorders / chemically induced
  • Cognition Disorders / drug therapy*
  • Cognition Disorders / etiology
  • Conditioning, Operant / drug effects
  • Dose-Response Relationship, Drug
  • Excitatory Amino Acid Antagonists / toxicity*
  • Exploratory Behavior / drug effects
  • Female
  • Half-Life
  • Learning / drug effects
  • Nicotinic Agonists / administration & dosage
  • Nicotinic Agonists / blood
  • Nicotinic Agonists / pharmacokinetics
  • Nicotinic Agonists / therapeutic use*
  • Phencyclidine / toxicity*
  • Random Allocation
  • Rats
  • Receptors, Nicotinic / metabolism*
  • Reversal Learning / drug effects
  • Schizophrenia / physiopathology*
  • Tachyphylaxis
  • alpha7 Nicotinic Acetylcholine Receptor

Substances

  • Benzamides
  • Bridged Bicyclo Compounds
  • Chrna7 protein, rat
  • Excitatory Amino Acid Antagonists
  • Nicotinic Agonists
  • PNU-282987
  • Receptors, Nicotinic
  • alpha7 Nicotinic Acetylcholine Receptor
  • Phencyclidine