Autocrine IL-6 regulates GRO-alpha production in thymic epithelial cells

Cytokine. 2010 Aug;51(2):195-201. doi: 10.1016/j.cyto.2010.05.002.

Abstract

Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-alpha (GRO-alpha) was subsequently expressed after IL-6 production. Either IL-6 or GRO-alpha was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-alpha production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-alpha production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-alpha and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autocrine Communication
  • Chemokine CXCL1 / biosynthesis*
  • Epithelial Cells / metabolism*
  • Humans
  • Interleukin-6 / immunology
  • Interleukin-6 / physiology*
  • Janus Kinase 2 / antagonists & inhibitors
  • Janus Kinase 2 / physiology*
  • Signal Transduction
  • Thymus Gland / metabolism*

Substances

  • CXCL1 protein, human
  • Chemokine CXCL1
  • Interleukin-6
  • JAK2 protein, human
  • Janus Kinase 2