A Ru(II)-TAP complex, photoreagent for tryptophan-containing peptides: structure of the covalent photoadduct

Inorg Chem. 2010 Aug 2;49(15):6796-8. doi: 10.1021/ic101151e.

Abstract

We report the first structure determination of a covalent photoadduct between a Ru(II)-tap complex and a tryptophan-containing peptide (AlaTrpAla) by mass spectrometry and NMR spectroscopy. Ru(II)-tap complexes could thus be exploited as photodamaging agents of Trp-containing polypeptides or proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Oligopeptides / chemistry*
  • Oligopeptides / metabolism*
  • Organometallic Compounds / chemistry*
  • Organometallic Compounds / metabolism*
  • Phenanthrenes / chemistry*
  • Phenanthrenes / metabolism*
  • Photochemical Processes*
  • Tryptophan / chemistry*
  • Tryptophan / metabolism*

Substances

  • Oligopeptides
  • Organometallic Compounds
  • Phenanthrenes
  • ruthenium-tris-1,4,5,8-tetraazaphenanthrene
  • Tryptophan