Development of single-tube mutiplex RT-PCR for dengue virus typing

Southeast Asian J Trop Med Public Health. 2009 Nov;40(6):1254-8.

Abstract

This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross Reactions
  • DNA Primers
  • Dengue / virology*
  • Dengue Virus / classification*
  • Dengue Virus / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Serotyping / methods*

Substances

  • DNA Primers