Abstract
A new intracellular mRNA imaging probe has been developed that incorporates thiol-terminated hairpin oligonucleotides covalently bound to the surface of citrate-capped gold nanoparticles. The hairpin DNA-coated gold nanoparticles (hAuNPs) positively identifies tyrosinase mRNA in cultured melanoma cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line, Tumor
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Gene Expression Regulation, Neoplastic*
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Gold / chemistry
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Humans
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Inverted Repeat Sequences*
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Melanoma / diagnosis*
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Melanoma / genetics
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Microscopy, Confocal / methods
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Microscopy, Fluorescence / methods
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Monophenol Monooxygenase / genetics*
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Nanoparticles* / chemistry
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Oligonucleotides* / chemistry
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RNA, Messenger / analysis*
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RNA, Messenger / genetics
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Sulfhydryl Compounds / chemistry
Substances
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Oligonucleotides
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RNA, Messenger
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Sulfhydryl Compounds
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Gold
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Monophenol Monooxygenase