Up-regulated Golgi phosphoprotein 2 (GOLPH2) expression in lung adenocarcinoma tissue

Clin Biochem. 2010 Aug;43(12):983-91. doi: 10.1016/j.clinbiochem.2010.05.010. Epub 2010 May 24.

Abstract

Objectives: The aim of the study was to evaluate Golgi Phosphoprotein 2 (GOLPH2) expression in lung cancer.

Design and methods: GOLPH2 expression was analyzed from 178 lung cancer tumor tissue samples using immunohistochemistry. Levels of 133 lung cancer patients' serum GOLPH2 (sGOLPH2) were also quantitatively compared with 70 healthy individuals by a sandwich enzyme-linked immunosorbent assay (ELISA).

Results: All adenocarcinoma (ADC) tissue samples (100%) displayed strong staining, 83.6% of which displayed cytoplasmic diffused staining. However, for squamous cell carcinoma samples, only 16.3% displayed strong staining intensity. Statistically, GOLPH2 expression in tissues of ADC was significantly higher than in other types of lung cancer (P<0.001). Levels of sGOLPH2 were also detected. The mean value of sGOLPH2 concentration is 69 ng/ml in lung cancer patients and 53 ng/ml in healthy individuals.

Conclusions: Significantly elevated GOLPH2 expression was observed in ADC tumor tissues. The levels of sGOLPH2 were about 30% higher in lung cancer patients compared with healthy individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Cell Line, Tumor
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Immunohistochemistry
  • In Vitro Techniques
  • Lung Neoplasms / metabolism*
  • Male
  • Membrane Proteins / metabolism*
  • Microscopy, Confocal
  • Middle Aged
  • Young Adult

Substances

  • GOLM1 protein, human
  • Membrane Proteins