Direct monitoring by carbon-13 nuclear magnetic resonance spectroscopy of the metabolism and metabolic rate of 13C-labeled compounds in vivo

Chem Pharm Bull (Tokyo). 1991 Jan;39(1):210-3. doi: 10.1248/cpb.39.210.

Abstract

Carbon-13 nuclear magnetic resonance spectroscopy has been used to observe the transformations of [1-13C]-D-glucose to [1,1'-13C2]-D-trehalose, and [3-13C]-L-alanine to [2-13C]-L-glutamic acid in the living body of Gryllodes sigillatus. [3-13C]-D-Alanine was not metabolized. The metabolic rate of [1-13C]-D-glucose was found to be altered by prior injection of boric acid.

MeSH terms

  • Alanine / metabolism*
  • Animals
  • Carbon Isotopes*
  • Glucose / metabolism*
  • Gryllidae / metabolism*
  • Magnetic Resonance Spectroscopy / methods*

Substances

  • Carbon Isotopes
  • Glucose
  • Alanine