RNA-based investigation of ammonia-oxidizing archaea in hot springs of Yunnan Province, China

Appl Environ Microbiol. 2010 Jul;76(13):4538-41. doi: 10.1128/AEM.00143-10. Epub 2010 Apr 30.

Abstract

Using RNA-based techniques and hot spring samples collected from Yunnan Province, China, we show that the amoA gene of aerobic ammonia-oxidizing archaea can be transcribed at temperatures higher than 74 degrees C and up to 94 degrees C, suggesting that archaeal nitrification can potentially occur at near boiling temperatures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ammonia / metabolism*
  • Archaea / enzymology*
  • Archaea / genetics
  • Archaea / isolation & purification
  • Archaea / metabolism
  • Archaeal Proteins / genetics
  • Archaeal Proteins / metabolism
  • China
  • Hot Springs / chemistry
  • Hot Springs / microbiology*
  • Hot Temperature
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Oxidoreductases / genetics
  • Oxidoreductases / metabolism*
  • RNA, Archaeal* / analysis
  • RNA, Archaeal* / genetics
  • RNA, Archaeal* / isolation & purification
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • Archaeal Proteins
  • RNA, Archaeal
  • RNA, Ribosomal, 16S
  • Ammonia
  • Oxidoreductases
  • ammonia monooxygenase

Associated data

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