Comparison of acid-induced inflammatory responses in the rat lung during high frequency oscillatory and conventional mechanical ventilation

Inflamm Res. 2010 Nov;59(11):931-7. doi: 10.1007/s00011-010-0204-6. Epub 2010 Apr 28.

Abstract

Background: The present study was performed to compare the effects of high frequency oscillatory ventilation (HFOV) with conventional mechanical ventilation (CMV) on pulmonary inflammatory responses in a rat acid-induced lung injury model.

Methods: Anesthetized rats were instilled intratracheally with HCl (0.1 N, 2 mL/kg) and then randomly divided into three mechanical ventilation settings: HFOV (an oscillatory frequency of 15 Hz, mean airway pressure (MAP) of 9 cmH(2)O), CMV at tidal volume of 12 and 6 mL/kg for 5 h.

Results: After HCl instillation, HFOV significantly attenuated the increases in neutrophil infiltration and TNF-α concentration in bronchoalveolar lavage fluid compared with the CMV groups. During HFOV, there was an inhibition of an increase in TNF-α mRNA expression and a decrease in SP-A mRNA expression induced by acid instillation.

Conclusion: This animal study demonstrates that HFOV is a suitable form of mechanical ventilation to prevent inflammatory responses in acid-induced lung injury.

MeSH terms

  • Animals
  • Bronchoalveolar Lavage Fluid / cytology
  • High-Frequency Ventilation*
  • Hydrochloric Acid / pharmacology
  • Inflammation / immunology*
  • Lung Injury / chemically induced
  • Lung Injury / immunology*
  • Lung Injury / therapy*
  • Male
  • Pulmonary Surfactant-Associated Protein A / genetics
  • Pulmonary Surfactant-Associated Protein A / metabolism
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Respiration, Artificial*
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Pulmonary Surfactant-Associated Protein A
  • Tumor Necrosis Factor-alpha
  • Hydrochloric Acid