Antifungal effects of the bioactive compounds enniatins A, A(1), B, B(1)

G Meca; J M Soriano; A Gaspari; A Ritieni; A Moretti; J Mañes

doi:10.1016/j.toxicon.2010.04.013

Antifungal effects of the bioactive compounds enniatins A, A(1), B, B(1)

Toxicon. 2010 Sep 1;56(3):480-5. doi: 10.1016/j.toxicon.2010.04.013. Epub 2010 Apr 24.

Authors

G Meca¹, J M Soriano, A Gaspari, A Ritieni, A Moretti, J Mañes

Affiliation

¹ Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of València, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain. giuseppe.meca@uv.es

PMID: 20417654
DOI: 10.1016/j.toxicon.2010.04.013

Abstract

To produce enniatin (ENs), Fusarium tricinctum CECT 20150 was grown in a liquid medium of potato (PDB), being mycotoxin purified by high performance liquid chromatography (HPLC) with a reverse phase semipreparative column using a mobile phase of acetonitrile/water using gradient condition. The purity of the ENs fractions was verified by analytical HPLC and LC/MS-MS. The pure fractions of ENs were utilized to study the biological activity on several mycotoxigenic moulds as Fusarium verticilloides, Fusarium sporotrichioides, Fusarium tricinctum, Fusarium poae, Fusarium oxysporum, Fusarium proliferatum, Beauveria bassiana, Trichoderma harzianum, Aspergillus flavus, Aspergillus parasiticus, Aspergillus fumigatus, Aspergillus ochraceus and Penicillium expansum. The results obtained demonstrated that in several antibiograms, ENs induced the inhibition of the grown microorganisms tested.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antifungal Agents / pharmacology*
Chromatography, High Pressure Liquid
Depsipeptides / pharmacology*
Microbial Sensitivity Tests
Tandem Mass Spectrometry

Substances

Antifungal Agents
Depsipeptides