Quantification of urinary PGEm, 6-keto PGF(1alpha) and 2,3-dinor-6-keto PGF(1alpha) by UFLC-MS/MS before and after exercise

Prostaglandins Other Lipid Mediat. 2010 Sep;93(1-2):8-13. doi: 10.1016/j.prostaglandins.2010.04.002. Epub 2010 Apr 21.

Abstract

Eicosanoids play an important role in the evaluation of pro-inflammatory responses and in the safety and toxicity of novel therapeutic agents. This work describes a high-throughput UFLCMS/MS method for the analysis of three urinary prostanoid biomarkers of pro-inflammatory responses, tetranor PGEm, 6-keto PGF(1alpha) and 2,3-dinor-6-keto PFG(1alpha). Nine male volunteers of various age and fitness level participated in this study. Six provided pre- and post-exercise samples and three provided intraday samples. Tetranor PGEm and 6-keto PGF(1alpha) increased significantly in patients after exercise (p<0.017 and p<0.029). In individual patient sets, tetranor PGEm levels increased from 1.5- to 6-fold pre- vs. post-exercise, levels of 6-keto PGF(1alpha) increased more dramatically from 2- to 55-fold pre- vs. post-exercise. The prostanoid 2,3-dinor-6-keto PGF(1alpha) remained unchanged post-exercise. Data was normalized to urinary creatinine concentration, which increased approximately 40% post-exercise.

MeSH terms

  • 6-Ketoprostaglandin F1 alpha / analogs & derivatives*
  • 6-Ketoprostaglandin F1 alpha / urine*
  • Adult
  • Chromatography, Liquid*
  • Creatinine / urine
  • Exercise*
  • Humans
  • Male
  • Middle Aged
  • Prostaglandins / urine*
  • Tandem Mass Spectrometry*

Substances

  • Prostaglandins
  • tetranor prostaglandin M
  • 6-Ketoprostaglandin F1 alpha
  • 2,3-dinor-6-ketoprostaglandin F1alpha
  • Creatinine