Antibacterial effect of the bioactive compound beauvericin produced by Fusarium proliferatum on solid medium of wheat

G Meca; I Sospedra; J M Soriano; A Ritieni; A Moretti; J Mañes

doi:10.1016/j.toxicon.2010.03.022

Antibacterial effect of the bioactive compound beauvericin produced by Fusarium proliferatum on solid medium of wheat

Toxicon. 2010 Sep 1;56(3):349-54. doi: 10.1016/j.toxicon.2010.03.022. Epub 2010 Apr 3.

Authors

G Meca¹, I Sospedra, J M Soriano, A Ritieni, A Moretti, J Mañes

Affiliation

¹ Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain. giuseppe.meca@uv.es

PMID: 20371252
DOI: 10.1016/j.toxicon.2010.03.022

Abstract

To obtain the bioactive compound beauvericin (BEA), Fusarium proliferatum CECT 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (SSF), being this mycotoxin purified by high performance liquid chromatography (HPLC) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. The purity of the BEA was verified by analytical HPLC and liquid chromatography tandem mass spectrometry (LC/MS-MS). The pure fractions of BEA were utilized to determinate the antibiotic effects on several bacterial strains that are considered normally pathogens of the intestinal tract as: Escherichia coli, Enterococcus faecium, Salmonella enterica, Shigella dysenteriae, Listeria monocytogenes, Yersinia enterocolitica, Clostridium perfringens, Pseudomonas aeruginosa and Staphylococcus aureus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Chromatography, Liquid
Depsipeptides / pharmacology*
Fusarium / metabolism*
Spectrometry, Mass, Electrospray Ionization
Spectrophotometry, Ultraviolet
Tandem Mass Spectrometry
Triticum / metabolism*
Triticum / microbiology

Substances

Anti-Bacterial Agents
Depsipeptides
beauvericin