Abstract
GBS (Group B Streptococcus) requires capsular Sia (sialic acid) for virulence and partially modifies this sugar by O-acetylation. In the present paper we describe serotype-specific patterns of GBS Sia O-acetylation that can be manipulated by genetic and biochemical means. In vitro and in vivo assays demonstrate that this subtle modification attenuates GBS Sia-mediated neutrophil suppression and animal virulence.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acetylation
-
Animals
-
Humans
-
Kinetics
-
Male
-
Mice
-
N-Acetylneuraminic Acid / genetics
-
N-Acetylneuraminic Acid / metabolism*
-
Neutrophils / immunology*
-
Neutrophils / metabolism
-
Polysaccharides, Bacterial / genetics
-
Polysaccharides, Bacterial / immunology
-
Polysaccharides, Bacterial / metabolism
-
Streptococcus agalactiae / genetics
-
Streptococcus agalactiae / immunology*
-
Streptococcus agalactiae / metabolism
-
Streptococcus agalactiae / pathogenicity*
-
Virulence / genetics
-
Virulence / physiology*
Substances
-
Polysaccharides, Bacterial
-
N-Acetylneuraminic Acid