Characterization of various promoter regions of the human DNA helicase-encoding genes and identification of duplicated ets (GGAA) motifs as an essential transcription regulatory element

Exp Cell Res. 2010 May 15;316(9):1523-34. doi: 10.1016/j.yexcr.2010.03.009. Epub 2010 Mar 17.

Abstract

DNA helicases are important in the regulation of DNA transaction and thereby various cellular functions. In this study, we developed a cost-effective multiple DNA transfection assay with DEAE-dextran reagent and analyzed the promoter activities of the human DNA helicases. The 5'-flanking regions of the human DNA helicase-encoding genes were isolated and subcloned into luciferase (Luc) expression plasmids. They were coated onto 96-well plate and used for co-transfection with a renilla-Luc expression vector into various cells, and dual-Luc assays were performed. The profiles of promoter activities were dependent on cell lines used. Among these human DNA helicase genes, XPB, RecQL5, and RTEL promoters were activated during TPA-induced HL-60 cell differentiation. Interestingly, duplicated ets (GGAA) elements are commonly located around the transcription start sites of these genes. The duplicated GGAA motifs are also found in the promoters of DNA replication/repair synthesis factor genes including PARG, ATR, TERC, and Rb1. Mutation analyses suggested that the duplicated GGAA-motifs are necessary for the basal promoter activity in various cells and some of them positively respond to TPA in HL-60 cells. TPA-induced response of 44-bp in the RTEL promoter was attenuated by co-transfection of the PU.1 expression vector. These findings suggest that the duplicated ets motifs regulate DNA-repair associated gene expressions during macrophage-like differentiation of HL-60 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Blotting, Western
  • Carcinogens / pharmacology
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • DEAE-Dextran
  • DNA Helicases / genetics*
  • DNA Helicases / metabolism*
  • Humans
  • Luciferases / metabolism
  • Macrophages / cytology
  • Macrophages / metabolism
  • Promoter Regions, Genetic / genetics*
  • Proto-Oncogene Proteins c-ets / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Regulatory Elements, Transcriptional / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transcriptional Activation
  • Transfection
  • Tretinoin / pharmacology

Substances

  • Antineoplastic Agents
  • Carcinogens
  • Proto-Oncogene Proteins c-ets
  • RNA, Messenger
  • Tretinoin
  • DEAE-Dextran
  • Luciferases
  • DNA Helicases
  • Tetradecanoylphorbol Acetate