Nonradioactive in situ hybridization. A rapid approach for the identification of marker chromosomes: study of a case of acute leukemia with a Yq specific DNA probe

Cancer Genet Cytogenet. 1991 Apr;52(2):165-72. doi: 10.1016/0165-4608(91)90459-8.

Abstract

Nonradioactive in situ hybridization provides a rapid method for detecting specific nucleic acid sequences. In this study of a patient with acute leukemia, we applied in situ hybridization for identification of a marker chromosome and determination of the number of copies of this marker in interphase nuclei using a biotinylated Yq-specific DNA probe (pY3.4). We show that nonradioactive interphase in situ hybridization can be a useful method for karyotypic analysis in addition to routine cytogenetic techniques in neoplastic disorders.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acute Disease
  • Aged
  • Chromosome Banding
  • DNA Probes
  • Genetic Markers
  • Humans
  • Karyotyping
  • Leukemia / genetics*
  • Male
  • Metaphase
  • Nucleic Acid Hybridization
  • Y Chromosome*

Substances

  • DNA Probes
  • Genetic Markers