Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope

J Biomed Opt. 2010 Jan-Feb;15(1):016018. doi: 10.1117/1.3309743.

Abstract

We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of oblique-incidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm x 4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solution-phase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biotin / metabolism
  • High-Throughput Screening Assays / instrumentation
  • High-Throughput Screening Assays / methods*
  • Humans
  • Immunoglobulin G / metabolism
  • Jurkat Cells
  • Mice
  • Microscopy, Fluorescence / methods*
  • Protein Array Analysis / methods*
  • Small Molecule Libraries / metabolism
  • Streptavidin / metabolism

Substances

  • Immunoglobulin G
  • Small Molecule Libraries
  • Biotin
  • Streptavidin