Abstract
ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization. The variety of biological functions dependent on ISWI suggests that its activity could be highly regulated. Our group has previously isolated and characterized new cellular activities that positively regulate ISWI in Drosophila melanogaster. To identify factors that antagonize ISWI activity we developed a novel in vivo eye-based assay to screen for genetic suppressors of ISWI. Our screen revealed that ISWI interacts with an evolutionarily conserved network of cellular and nuclear factors that escaped previous genetic and biochemical analyses.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenosine Triphosphatases / antagonists & inhibitors
-
Adenosine Triphosphatases / genetics
-
Adenosine Triphosphatases / metabolism*
-
Animals
-
Biological Assay
-
Cell Cycle
-
Conserved Sequence*
-
Drosophila Proteins / genetics
-
Drosophila Proteins / metabolism*
-
Drosophila melanogaster / cytology
-
Drosophila melanogaster / genetics
-
Drosophila melanogaster / metabolism*
-
Evolution, Molecular*
-
Eye / pathology
-
Fluorometry
-
Genes, Suppressor
-
Genetic Testing
-
Mutation / genetics
-
Nucleosomes / metabolism*
-
Phenotype
-
Protein Binding
-
Transcription Factors / antagonists & inhibitors
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
Substances
-
Drosophila Proteins
-
ISWI protein
-
Nucleosomes
-
Transcription Factors
-
Adenosine Triphosphatases