Induction of apoptosis by [8]-shogaol via reactive oxygen species generation, glutathione depletion, and caspase activation in human leukemia cells

J Agric Food Chem. 2010 Mar 24;58(6):3847-54. doi: 10.1021/jf904563c.

Abstract

Ginger, the rhizome of Zingiber officinale , is a traditional medicine with a carminative effect and antinausea, anti-inflammatory, and anticarcinogenic properties. This study examined the growth inhibitory effects of [8]-shogaol, one of the pungent phenolic compounds in ginger, on human leukemia HL-60 cells. It demonstrated that [8]-shogaol was able to induce apoptosis in a time- and concentration-dependent manner. Treatment with [8]-shogaol caused a rapid loss of mitochondrial transmembrane potential, stimulation of reactive oxygen species (ROS) production, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 and procaspase-3 processing. Taken together, these results suggest for the first time that ROS production and depletion of glutathione that contributed to [8]-shogaol-induced apoptosis in HL-60 cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects*
  • Caspase 3 / metabolism*
  • Caspase 9 / metabolism*
  • Catechols / pharmacology*
  • Cell Line, Tumor
  • Enzyme Activation / drug effects
  • Glutathione / metabolism
  • Humans
  • Leukemia / enzymology
  • Leukemia / metabolism
  • Leukemia / physiopathology*
  • Plant Extracts / pharmacology*
  • Reactive Oxygen Species / metabolism*
  • Zingiber officinale / chemistry

Substances

  • Catechols
  • Plant Extracts
  • Reactive Oxygen Species
  • shogaol
  • Caspase 3
  • Caspase 9
  • Glutathione