Development of Cryptosporidium parvum-induced gastrointestinal neoplasia in severe combined immunodeficiency (SCID) mice: severity of lesions is correlated with infection intensity

Am J Trop Med Hyg. 2010 Feb;82(2):257-65. doi: 10.4269/ajtmh.2010.09-0309.

Abstract

We reported previously that Cryptosporidium parvum was able to induce intestinal tumors in severe combined immunodeficiency (SCID) mice treated with corticoids. To further characterize this Cryptosporidium-induced cell transformation, SCID mice treated with dexamethasone were challenged with C. parvum oocysts, and euthanatized sequentially after infection for histologic examination. Ki-67 was used as a marker of cellular proliferation. Our previous results were confirmed, and it was also found that mice receiving higher inocula (10(6)-10(7)) experienced more severe neoplastic development. Additionally, neoplastic changes were observed not only in the caecum but also in the stomach and duodenum of some animals. Interestingly, SCID mice (6/6) inoculated with 10(5)-10(7) oocysts showed high grade intraepithelial neoplasia or adenomas with high grade dysplasia in the caecum after Day 46 post-infection (PI). Immunohistochemistry for Ki-67 staining indicated the neoplastic process associated to cryptosporidiosis, and evidenced the first immunohistochemical alterations at early stages of the process, even at 3 weeks PI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cryptosporidiosis / complications*
  • Cryptosporidiosis / parasitology
  • Cryptosporidium parvum*
  • Dexamethasone / pharmacology
  • Gastrointestinal Neoplasms / complications*
  • Gastrointestinal Neoplasms / parasitology
  • Gene Expression Regulation
  • Immunosuppressive Agents / pharmacology
  • Ki-67 Antigen / metabolism
  • Mice
  • Mice, SCID

Substances

  • Immunosuppressive Agents
  • Ki-67 Antigen
  • Mki67 protein, mouse
  • Dexamethasone