Abstract
A comparison between conventional identification and 16S rRNA gene sequencing of anaerobic bacteria isolated from blood cultures in a routine setting was performed (n = 127). With sequencing, 89% were identified to the species level, versus 52% with conventional identification. The times for identification were 1.5 days and 2.8 days, respectively.
Publication types
-
Comparative Study
-
Evaluation Study
MeSH terms
-
Bacteria, Anaerobic / classification
-
Bacteria, Anaerobic / genetics
-
Bacteria, Anaerobic / isolation & purification*
-
Bacterial Infections / diagnosis
-
Bacteriological Techniques / methods*
-
Blood / microbiology*
-
DNA, Bacterial / chemistry
-
DNA, Bacterial / genetics*
-
DNA, Ribosomal / chemistry
-
DNA, Ribosomal / genetics*
-
Humans
-
RNA, Ribosomal, 16S / genetics*
-
Sensitivity and Specificity
-
Sequence Analysis, DNA / methods*
-
Time Factors
Substances
-
DNA, Bacterial
-
DNA, Ribosomal
-
RNA, Ribosomal, 16S