A benchtop, ultrafast infrared spectroscopic imaging system for biomedical applications

Rev Sci Instrum. 2009 Dec;80(12):123702. doi: 10.1063/1.3262499.

Abstract

We discuss the potential biomedical applications for a novel infrared spectroscopic microimaging system. A tunable, table top solid-state laser has been coupled to a commercial infrared microscope, fitted with a modified high resolution infrared camera, to create a unique tool for midinfrared imaging. The system is capable of performing broadband imaging at a diffraction-limited spatial resolution, as is demonstrated here by spatially resolved spectroscopy of polymer test samples with a spectral resolution of 20 cm(-1). The large pulse energies (tens of microjoules) offer previously unobtainable combinations of high signal-to-noise levels and rapid data collection times which are superior to current stand-alone laboratory instruments by many decades. Coupled with the short (100 ps) short pulse duration, these characteristics promise to make a wide range of time-resolved and reflection mode imaging experiments possible with live biological systems.

MeSH terms

  • Calibration
  • Copper
  • Electronics, Medical / instrumentation*
  • Lasers
  • Microscopy / instrumentation*
  • Microscopy, Electron
  • Optics and Photonics / instrumentation
  • Polymers
  • Polymethyl Methacrylate
  • Silicon Compounds
  • Spectroscopy, Fourier Transform Infrared / instrumentation*
  • Time Factors

Substances

  • Polymers
  • Silicon Compounds
  • Copper
  • Polymethyl Methacrylate