Abstract
Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Electrophoresis, Polyacrylamide Gel
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Intramolecular Transferases / biosynthesis*
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Intramolecular Transferases / genetics
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Intramolecular Transferases / isolation & purification*
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Intramolecular Transferases / metabolism
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Kinetics
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Oryza / enzymology*
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Recombinant Proteins / biosynthesis*
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification*
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Recombinant Proteins / metabolism
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Spodoptera / cytology
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Spodoptera / genetics*
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Uridine Diphosphate Sugars / metabolism*
Substances
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Recombinant Proteins
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Uridine Diphosphate Sugars
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uridine diphosphate arabinose
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Intramolecular Transferases