A storage protein-like Trypsin inhibitor from the Moth Bean (Phaseolus acutifolius) with antiproliferative activity toward lymphoma cells

Protein Pept Lett. 2010 Jun;17(6):782-8. doi: 10.2174/092986610791190408.

Abstract

A 26-kDa trypsin inhibitor with an N-terminal sequence resembling storage proteins was purified from moth beans (Phaseolus acutifolius). It dose-dependently inhibited trypsin with an IC(50) value of about 0.38 microM. It inhibited [methyl-(3)H] thymidine incorporation by lymphoma MBL2 cells with an IC(50) value of 20 microM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antifungal Agents / chemistry
  • Antifungal Agents / metabolism
  • Antifungal Agents / pharmacology
  • Botrytis / drug effects
  • Botrytis / growth & development
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Dose-Response Relationship, Drug
  • Electrophoresis, Polyacrylamide Gel
  • HIV Reverse Transcriptase / antagonists & inhibitors
  • HIV Reverse Transcriptase / metabolism
  • Humans
  • Lymphoma / metabolism
  • Lymphoma / pathology
  • Molecular Sequence Data
  • Molecular Weight
  • Phaseolus / metabolism*
  • Plant Proteins / chemistry
  • Plant Proteins / metabolism
  • Plant Proteins / pharmacology*
  • Reverse Transcriptase Inhibitors / chemistry
  • Reverse Transcriptase Inhibitors / metabolism
  • Reverse Transcriptase Inhibitors / pharmacology
  • Thymidine / metabolism
  • Tritium
  • Trypsin / metabolism
  • Trypsin Inhibitors / chemistry
  • Trypsin Inhibitors / metabolism
  • Trypsin Inhibitors / pharmacology*

Substances

  • Antifungal Agents
  • Plant Proteins
  • Reverse Transcriptase Inhibitors
  • Trypsin Inhibitors
  • Tritium
  • HIV Reverse Transcriptase
  • Trypsin
  • Thymidine