Engineering the mouse genome to model human disease for drug discovery

Frank Koentgen; Gabriele Suess; Dieter Naf

doi:10.1007/978-1-60761-058-8_4

Engineering the mouse genome to model human disease for drug discovery

Methods Mol Biol. 2010:602:55-77. doi: 10.1007/978-1-60761-058-8_4.

Authors

Frank Koentgen¹, Gabriele Suess, Dieter Naf

Affiliation

¹ Ozgene Pty. Ltd., Bentley DC, WA, Australia.

PMID: 20012392
DOI: 10.1007/978-1-60761-058-8_4

Abstract

Genetically engineered mice (GEM) have become invaluable tools for human disease modeling and drug development. Completion of the mouse genome sequence in combination with transgenesis and gene targeting in embryonal stem cells have opened up unprecedented opportunities. Advanced technologies for derivation of GEM models will be introduced and discussed.

MeSH terms

Animals
Disease Models, Animal*
Drug Discovery*
Embryonic Stem Cells / physiology
Gene Knock-In Techniques
Gene Targeting / methods
Gene Transfer Techniques*
Genetic Engineering / methods
Genome*
Humans
Mice
Mice, Transgenic*
RNA Interference