Characterization of MSCs from human placental decidua basalis in hypoxia and serum deprivation

Cell Biol Int. 2010 Jan 27;34(3):237-43. doi: 10.1042/CBI20090044.

Abstract

Recently, we reported that human PDB (placental decidua basalis) is an excellent source of MSCs (mesenchymal stem cells), meanwhile, PDB-MSCs could survive under hypoxia and serum deprivation. Herein, we investigated the proliferation, clonogentic efficiency, phenotypes, metabolic activity and cytokines secretion of PDB-MSCs in hypoxia and serum deprivation. PDB-MSCs were cultured in four groups: normoxia (20% O2) and complete medium [10% FBS (foetal bovine serum)+DMEM-HG (Dulbecco's modified Eagle's medium-high glucose)], hypoxia and complete medium, normoxia and serum deprivation (0% FBS), and hypoxia and serum deprivation. After 96 h of culture in the above groups, PDB-MSCs maintain the phenotypes stably. Interestingly, hypoxia notably enhanced the proliferation, colony-forming potential and lactate/glucose ratio in complete medium, but suppressed the secretion of BMP-2 (bone morphogenetic protein-2) and bFGF (basic fibroblast growth factor), while it did not change the quantity of VEGF (vascular endothelial growth factor) and bFGF in serum deprivation. Although PDB-MSCs grew slowly and seldom formed a colony unit in hypoxia and serum deprivation, they possessed a moderate metabolism. In conclusion, our results indicate that PDB-MSCs appear to be promising seed cells for ischaemia-related tissue engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Morphogenetic Protein 2 / metabolism
  • Cell Hypoxia
  • Cell Proliferation
  • Culture Media, Serum-Free
  • Decidua / cytology*
  • Female
  • Fibroblast Growth Factor 2 / metabolism
  • Flow Cytometry
  • Humans
  • Immunophenotyping
  • Mesenchymal Stem Cells / cytology*
  • Phenotype
  • Placenta / cytology*
  • Pregnancy
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Culture Media, Serum-Free
  • Vascular Endothelial Growth Factor A
  • Fibroblast Growth Factor 2