Abstract
DNA methylation is an epigenetic mark affecting genes and transposons. Screening for mutants that fail to establish DNA methylation yielded two we termed "involved in de novo" (idn) 1 and 2. IDN1 encodes DMS3, an SMC-related protein, and IDN2 encodes a previously unknown double-stranded RNA-binding protein with homology to SGS3. IDN1 and IDN2 control de novo methylation and small interfering RNA (siRNA)-mediated maintenance methylation and are components of the RNA-directed DNA methylation pathway.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Arabidopsis / physiology*
-
Arabidopsis Proteins / genetics
-
Arabidopsis Proteins / physiology*
-
Chromosomal Proteins, Non-Histone / genetics
-
Chromosomal Proteins, Non-Histone / physiology*
-
DNA Methylation*
-
RNA-Binding Proteins / genetics
-
RNA-Binding Proteins / physiology*
Substances
-
Arabidopsis Proteins
-
Chromosomal Proteins, Non-Histone
-
DMS3 protein, Arabidopsis
-
IDN2 protein, Arabidopsis
-
RNA-Binding Proteins